Advanced Microscopy

Joaquim Grego

Introduction

The Advanced Microscopy Platform of the Josep Carreras Leukaemia Research Institute offers cutting-edge services in fluorescence (laser and conventional light source), bright field microscopy and High-performance Slide Scanner and Image analysis. The Platform offers a unique opportunity for internal and external researchers to take advantage of advanced technologies without incurring costly investments in equipment, with a wide operational availability, from 9 a.m. to 7 p.m. with technical assistance, and 24/7 for trained, self-sufficient users. The Unit also offers Comprehensive Support to solve problems, provides technical advice and personalized assistance. The guarantee of a complete service is extended to our external users. Should you need it, the Unit provides Specialized Training programs allowing users to be autonomous in the use of the equipment. This initiative ensures that both internal and external users get the most out of the Unit’s facilities.

Applications: Molecular biology, histopathology, microbiology, organoids, 3D imaging.

Services

CONFOCAL MICROSCOPY

Leica Stellaris 8

• WLL white laser; up to 8 excitation lines between 440-790 nm. You can work with any fluorochrome excited in this range and ensure maximum excitation efficiency.

• Solid state laser 405 nm.

• TauSense – rapid analysis of fluorescence lifetimes. Allows the separation of fluorochromes and auto-fluorescence.

• Spectral Detectors – 4 high sensitivity and contrast HyD S detectors for general applications.

• Super-resolution module: confocal images up to 100 nm XY resolution.

• Galvometric (high quality up to 8096 x 8096) and Resonant (8000 lines/second 1024 x 1024, 28 images/second 512 x 512) duo scanning.

• Leica AFC (Adaptive Focus Control) focus correction system

• Motorized stage for mosaic and multi-position experiments.

• Objectives:

HC PL APO 10x/0.40 W.D. 2.2mm (dry)
HC PL FLUOTAR 20x/0.75 W.D. 0.62mm (dry)
HC FLUOTAR L 25x/0.95 W VISIR (water) Long working distance, for thick specimens HC PL APO 40x/1.25 CORR CS2 (Glycerol). Long working distance, thick specimens
HC PL APO 40x/1.30 CS2 (oil)
PL APO 63x/1.40 (oil)

HIGH PERFORMANCE SCANNING FOR BRIGHTFIELD AND FLUORESCENT SAMPLES

Zeiss Axioscan 7

• High-performance Slide Scanner for Fluorescence (from 405nm to 647nm) and Brightfield

• Z-stack scanning.

• 100 slides in a single run.

EPIFLUORESCENCE (CONVENTIONAL) AND BRIGHTFIELD MICROSCOPY

Leica DM4

• Epifluorescence and brightfield microscopy.

• Objectives: 5X, 10X, 20X, 40X and 63X dry objectives. 100X oil immersion

Olympus BX23

• High quality brightfield for clinical sample analysis.

• Motorized Stage.